Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution

Sumeyra Alkis Kocturk; Derin Nabaz Fisal Fisal; Hakki Alma

doi:doi:10.11648/j.ajlm.20210602.13

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Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution

Sumeyra Alkis Kocturk, Derin Nabaz Fisal Fisal, Hakki Alma

Published in American Journal of Laboratory Medicine (Volume 6, Issue 2)

Received: 6 January 2021 Accepted: 3 May 2021 Published: 14 May 2021

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Abstract

The aim at the current study was to determine that essential oils of mastic gum Pistacia khinjuk has some antibacterial activity. The essential oil was obtained with two methods (conventional and microwave method) for obtanining aqeous extract of gum essential oils with some biological activities from Pistacia khinjuk gum of Iraqıan. The analysis of highest essential oil yield of the gum for microwave method was in 200W for 90 min and the lowest yield in 500W for 60 min while in conventional method the highest yield was in 90 min and lowest was in 30 min. obtained from the study made in another laboratory in same university. Moreover, the analysis of physicochemical and phytochemical components of Pistacia khinjuk gum essentials oil by GC-MS in my colleaques’ research in Natural and Applied Sciences field. The main compounds were (+)-α-Pinene, (±)-β-Pinene, D- Limonene and Camphene. The antibacterial activity of essential oil provided by two methods were compared against different species of bacteria. The results of the antimicrobial activity tests (minimal inhibitory concentration (MIC) and agar-disk diffusion) indicate that essential oils of mastic gum Pistacia khinjuk provided by both methods have higher activity against the tested strains compared to Gentamicin and Ampicilin, confirming its traditional uses. Moreover, mastic gum oil was found to inhibit both gram-positive; Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Listeria monocytogenes ATCC 15313 and Bacillus cereus ATCC 23456 and gram-negative bacteria; Salmonella enterica NTCT 13, E coli O157:H7 ATCC 35130, Klebsiella pneumonia ATCC 700603 with the exception of Chronobactersakazakii ATCC 29544.

Published in	American Journal of Laboratory Medicine (Volume 6, Issue 2)
DOI	10.11648/j.ajlm.20210602.13
Page(s)	27-30
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Essential Oil, Pistacia Khinjuk Stoks Gum of IRAQIAN, Antibacterial Activity, Standart Strain

References

[1]	Fisal Fisal D. N. Chemical composition and some biological activities of essential oil from gum of Iraqian Pistacia khinjuk Stocks. Master thesis, Department of Forest Industry Engineering (2016).
[2]	Bozorgi M., Memariani Z., Mobli M., et al. Five Pistacia species (P. vera, P. atlantica, P. terebinthus, P. khinjuk, and P. lentiscus): A Review of Their Traditional Uses, Phytochemistry, and Pharmacology. Hindawi Publishing Corporation The Scientific World Journal - Volume 2013, Article ID 219815, 33 pages.
[3]	Al-Habbal, M. J.; Al-Habbal, Z.; Huwez, F. U. A double-blind controlled clinical trial of mastic and placebo in the treatment of duodenal ulcer. J. Clin. Exp. Pharmacol. Physiol. 1984, 11 (5), 541-4.
[4]	Huwez, F. U.; et al. Mastic gum kills Helicobacter pylori. N. Engl. J. Med. 1998, 339, 1946.
[5]	Iauk, L.; Ragusa, S.; Rapisadra, A., et al. In vitro antimicrobial activity of Pistacia lentiscus L. extracts: Preliminary report. J. Chemother. 1996, 8 (3), 207-9.
[6]	Klancnik A., et al. In Vitro Antimicrobial and Antioxidant Activity of Commercial Rosemary Extract Formulations. Journal of Food Protection, Vol. 72, No. 8, 2009, Pages 1744–1752.
[7]	Tan, M. C., Tan, C. P., and Ho, C. W., 2013. Effects of extraction solvent system, time and temperature on total phenolic content of henna (Lawsoniainermis) stems. International Food Research Journal, 20 (6): 3117-3123.
[8]	Taran M., et al. Antimicrobial activity of essential oil of Ferulago Angulata subsp. International Journal of Food Properties, 21:1, 173-185.
[9]	Tassou, C. C., Nychas, G. J. E., 1995. Antimicrobial activity of the essential oil of mastic gum (Pistacialentiscus var. chia) on gram positive and gram negative bacteria in broth and in model food system. Int. Biodeter. Biodegrad., 36, 411–420.
[10]	Magiatis, P.; Melliou, E.; Skaltsounis, A. L., et al. Chemical composition and antimicrobial activity of the essential oils of Pistacia lentiscus var. chia. Planta Medica, 65(8): 749-752.
[11]	Vasslios, P., Papageorgiou, A. N., Mellidis, A. S.., 1991 The Chemical Composition of the Essential Oil of Mastic Gum. J. Essent. Oil Res, 3, 107-110.
[12]	Özçelik, B., Aslan, M., Orhan, I., Karaoglu, T., 2005. Antibacterial, antifungal, and antiviral activities of the lipophylic extracts of Pistacia Vera. Microbiol. Res, 160: 159-164.
[13]	Watson, R. R., 2014. Polyphenols in Plants: Isolation, Purification and Extract Preparation. 1st ed., Elsevier Inc., USA.
[14]	Tsokou, A., Georgopoulou, K., Melliou, E., Magiatis, P., Tsitsa, E., 2007. Composition and enantiomeric analysis of the essential oil of thefruits and the leaves of Pistacia vera from Greece. Molecules, 12:1233-1239.
[15]	Voravuthikunchai, S. P., and Mitchell, H., 2008. Inhibitory and killing activities of medicinal plants against multiple antibiotic-resistant Helicobacter pylori. Journal of Health Science, 54 (1): 81-88.

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Sumeyra Alkis Kocturk, Derin Nabaz Fisal Fisal, Hakki Alma. (2021). Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution. American Journal of Laboratory Medicine, 6(2), 27-30. https://doi.org/10.11648/j.ajlm.20210602.13

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ACS Style

Sumeyra Alkis Kocturk; Derin Nabaz Fisal Fisal; Hakki Alma. Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution. Am. J. Lab. Med. 2021, 6(2), 27-30. doi: 10.11648/j.ajlm.20210602.13

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AMA Style

Sumeyra Alkis Kocturk, Derin Nabaz Fisal Fisal, Hakki Alma. Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution. Am J Lab Med. 2021;6(2):27-30. doi: 10.11648/j.ajlm.20210602.13

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@article{10.11648/j.ajlm.20210602.13,
  author = {Sumeyra Alkis Kocturk and Derin Nabaz Fisal Fisal and Hakki Alma},
  title = {Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution},
  journal = {American Journal of Laboratory Medicine},
  volume = {6},
  number = {2},
  pages = {27-30},
  doi = {10.11648/j.ajlm.20210602.13},
  url = {https://doi.org/10.11648/j.ajlm.20210602.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20210602.13},
  abstract = {The aim at the current study was to determine that essential oils of mastic gum Pistacia khinjuk has some antibacterial activity. The essential oil was obtained with two methods (conventional and microwave method) for obtanining aqeous extract of gum essential oils with some biological activities from Pistacia khinjuk gum of Iraqıan. The analysis of highest essential oil yield of the gum for microwave method was in 200W for 90 min and the lowest yield in 500W for 60 min while in conventional method the highest yield was in 90 min and lowest was in 30 min. obtained from the study made in another laboratory in same university. Moreover, the analysis of physicochemical and phytochemical components of Pistacia khinjuk gum essentials oil by GC-MS in my colleaques’ research in Natural and Applied Sciences field. The main compounds were (+)-α-Pinene, (±)-β-Pinene, D- Limonene and Camphene. The antibacterial activity of essential oil provided by two methods were compared against different species of bacteria. The results of the antimicrobial activity tests (minimal inhibitory concentration (MIC) and agar-disk diffusion) indicate that essential oils of mastic gum Pistacia khinjuk provided by both methods have higher activity against the tested strains compared to Gentamicin and Ampicilin, confirming its traditional uses. Moreover, mastic gum oil was found to inhibit both gram-positive; Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Listeria monocytogenes ATCC 15313 and Bacillus cereus ATCC 23456 and gram-negative bacteria; Salmonella enterica NTCT 13, E coli O157:H7 ATCC 35130, Klebsiella pneumonia ATCC 700603 with the exception of Chronobactersakazakii ATCC 29544.},
 year = {2021}
}

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TY  - JOUR
T1  - Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution
AU  - Sumeyra Alkis Kocturk
AU  - Derin Nabaz Fisal Fisal
AU  - Hakki Alma
Y1  - 2021/05/14
PY  - 2021
N1  - https://doi.org/10.11648/j.ajlm.20210602.13
DO  - 10.11648/j.ajlm.20210602.13
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 27
EP  - 30
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20210602.13
AB  - The aim at the current study was to determine that essential oils of mastic gum Pistacia khinjuk has some antibacterial activity. The essential oil was obtained with two methods (conventional and microwave method) for obtanining aqeous extract of gum essential oils with some biological activities from Pistacia khinjuk gum of Iraqıan. The analysis of highest essential oil yield of the gum for microwave method was in 200W for 90 min and the lowest yield in 500W for 60 min while in conventional method the highest yield was in 90 min and lowest was in 30 min. obtained from the study made in another laboratory in same university. Moreover, the analysis of physicochemical and phytochemical components of Pistacia khinjuk gum essentials oil by GC-MS in my colleaques’ research in Natural and Applied Sciences field. The main compounds were (+)-α-Pinene, (±)-β-Pinene, D- Limonene and Camphene. The antibacterial activity of essential oil provided by two methods were compared against different species of bacteria. The results of the antimicrobial activity tests (minimal inhibitory concentration (MIC) and agar-disk diffusion) indicate that essential oils of mastic gum Pistacia khinjuk provided by both methods have higher activity against the tested strains compared to Gentamicin and Ampicilin, confirming its traditional uses. Moreover, mastic gum oil was found to inhibit both gram-positive; Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Listeria monocytogenes ATCC 15313 and Bacillus cereus ATCC 23456 and gram-negative bacteria; Salmonella enterica NTCT 13, E coli O157:H7 ATCC 35130, Klebsiella pneumonia ATCC 700603 with the exception of Chronobactersakazakii ATCC 29544.
VL  - 6
IS  - 2
ER  -

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Author Information

Sumeyra Alkis Kocturk

Markasi Hospital, Kahramanmaras, Turkey
Derin Nabaz Fisal Fisal

Kahramanmaras Sutcu Imam University Forestry and Forest Engineering, Kahramanmaras, Turkey
Hakki Alma

Igdir University, Igdir, Turkey

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APA Style

Sumeyra Alkis Kocturk, Derin Nabaz Fisal Fisal, Hakki Alma. (2021). Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution. American Journal of Laboratory Medicine, 6(2), 27-30. https://doi.org/10.11648/j.ajlm.20210602.13

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ACS Style

Sumeyra Alkis Kocturk; Derin Nabaz Fisal Fisal; Hakki Alma. Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution. Am. J. Lab. Med. 2021, 6(2), 27-30. doi: 10.11648/j.ajlm.20210602.13

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AMA Style

Sumeyra Alkis Kocturk, Derin Nabaz Fisal Fisal, Hakki Alma. Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution. Am J Lab Med. 2021;6(2):27-30. doi: 10.11648/j.ajlm.20210602.13

Copy | Download

@article{10.11648/j.ajlm.20210602.13,
  author = {Sumeyra Alkis Kocturk and Derin Nabaz Fisal Fisal and Hakki Alma},
  title = {Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution},
  journal = {American Journal of Laboratory Medicine},
  volume = {6},
  number = {2},
  pages = {27-30},
  doi = {10.11648/j.ajlm.20210602.13},
  url = {https://doi.org/10.11648/j.ajlm.20210602.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajlm.20210602.13},
  abstract = {The aim at the current study was to determine that essential oils of mastic gum Pistacia khinjuk has some antibacterial activity. The essential oil was obtained with two methods (conventional and microwave method) for obtanining aqeous extract of gum essential oils with some biological activities from Pistacia khinjuk gum of Iraqıan. The analysis of highest essential oil yield of the gum for microwave method was in 200W for 90 min and the lowest yield in 500W for 60 min while in conventional method the highest yield was in 90 min and lowest was in 30 min. obtained from the study made in another laboratory in same university. Moreover, the analysis of physicochemical and phytochemical components of Pistacia khinjuk gum essentials oil by GC-MS in my colleaques’ research in Natural and Applied Sciences field. The main compounds were (+)-α-Pinene, (±)-β-Pinene, D- Limonene and Camphene. The antibacterial activity of essential oil provided by two methods were compared against different species of bacteria. The results of the antimicrobial activity tests (minimal inhibitory concentration (MIC) and agar-disk diffusion) indicate that essential oils of mastic gum Pistacia khinjuk provided by both methods have higher activity against the tested strains compared to Gentamicin and Ampicilin, confirming its traditional uses. Moreover, mastic gum oil was found to inhibit both gram-positive; Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Listeria monocytogenes ATCC 15313 and Bacillus cereus ATCC 23456 and gram-negative bacteria; Salmonella enterica NTCT 13, E coli O157:H7 ATCC 35130, Klebsiella pneumonia ATCC 700603 with the exception of Chronobactersakazakii ATCC 29544.},
 year = {2021}
}

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TY  - JOUR
T1  - Evaluation of Antibacterial Activity of Essential Oil from Pistacia Khinjuk Stoks Gum of Iraqian Using Broth Microdilution
AU  - Sumeyra Alkis Kocturk
AU  - Derin Nabaz Fisal Fisal
AU  - Hakki Alma
Y1  - 2021/05/14
PY  - 2021
N1  - https://doi.org/10.11648/j.ajlm.20210602.13
DO  - 10.11648/j.ajlm.20210602.13
T2  - American Journal of Laboratory Medicine
JF  - American Journal of Laboratory Medicine
JO  - American Journal of Laboratory Medicine
SP  - 27
EP  - 30
PB  - Science Publishing Group
SN  - 2575-386X
UR  - https://doi.org/10.11648/j.ajlm.20210602.13
AB  - The aim at the current study was to determine that essential oils of mastic gum Pistacia khinjuk has some antibacterial activity. The essential oil was obtained with two methods (conventional and microwave method) for obtanining aqeous extract of gum essential oils with some biological activities from Pistacia khinjuk gum of Iraqıan. The analysis of highest essential oil yield of the gum for microwave method was in 200W for 90 min and the lowest yield in 500W for 60 min while in conventional method the highest yield was in 90 min and lowest was in 30 min. obtained from the study made in another laboratory in same university. Moreover, the analysis of physicochemical and phytochemical components of Pistacia khinjuk gum essentials oil by GC-MS in my colleaques’ research in Natural and Applied Sciences field. The main compounds were (+)-α-Pinene, (±)-β-Pinene, D- Limonene and Camphene. The antibacterial activity of essential oil provided by two methods were compared against different species of bacteria. The results of the antimicrobial activity tests (minimal inhibitory concentration (MIC) and agar-disk diffusion) indicate that essential oils of mastic gum Pistacia khinjuk provided by both methods have higher activity against the tested strains compared to Gentamicin and Ampicilin, confirming its traditional uses. Moreover, mastic gum oil was found to inhibit both gram-positive; Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, Listeria monocytogenes ATCC 15313 and Bacillus cereus ATCC 23456 and gram-negative bacteria; Salmonella enterica NTCT 13, E coli O157:H7 ATCC 35130, Klebsiella pneumonia ATCC 700603 with the exception of Chronobactersakazakii ATCC 29544.
VL  - 6
IS  - 2
ER  -

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